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doi: 10.3168/jds.2020-19437 88 LiLFangZLiuXHuWLuWLeeYKet al

The membrane was blocked with 3% non-fat milk (BioRad, Hercules, CA, United States) at room temperature for 1.5 h, washed, and incubated with primary antibodies (Supplementary Table 3) at 4C overnight on a rotating shaker