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After incubation, the slides were subjected to electrophoresis at 25 V (0.8 V/cm over the platform, 300 mA) for 30 min at 4C

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During the earliest stages of embryo cleavage intracellular GSH levels are very low but the presence of other antioxidants and antioxidant enzymes, including superoxide dismutase and catalase, compensate for this to maintain redox balance [14,17,18]

This research project was financially supported by the National Key Research and Development Program of China (2022YFD1600304), the Project for Provincial Agricultural Science and Technology Innovation and Promotion in Guangdong (2023KJ142), the Scientific and Technological Project of Yunfu (2021020605), National Natural Science Foundation of China Youth Program (82304804) and Yunnan Science and Technology Talents and Platform Program (202205AF150071), Natural Science Foundation of Hunan Province of China (2024JJ7415), and Education Department of Hunan Province of China (24B0501)