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The recipient 018Z or Jurkat cells were cultured for 48 hours with lentivirus-containing medium supplemented with 8 g/ml Polybrene (Merck, # TR-1003) and then for additional 48 hours with fresh medium, before undergoing a 4-day selection in medium supplemented with 1.5g/mL puromycin (Thermo Fisher Scientific, # A1113903)

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Notably, the extent of migration enhancement by these EVs was similar to that induced by native FBS-containing EVs, indicating that EVs were responsible for the observed increase in cell migration (Supplementary Fig