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The pipeline bins the mapped reads into GATC-fragments according to GATC-sites indicated by a gff file for the dme_r6 fly genome (already provided by the authors of the pipeline on the website above) and normalizes reads against the Dam controls, in our case, the C929-Gal4/+

Therefore, Content of cysteine or cysteine precursors determines the glutathione level in neurons since neurons are not able to use the cystine but rather rely on the availability of cysteine for their glutathione synthesis [20]