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A high-throughput screening (HTS)-compatible thallium flux assay for G i/o -coupled GPCRs using GIRK channels as a readout was developed (Niswender et al., 2008)

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These enzymes are responsible for the metabolism of B(a)P to a carcinogenic compound BPDE [benzo(a)pyrene-7,8-diol-9,10-epoxide], and CYP induction is associated with damage to the DNA of spermatozoa and ROS overexpression ( Overall, the results presented here show how the use of Modified Risk Tobacco Products can induce some of the same toxicological outcomes triggered by tobacco smoking, although less marked