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qRT-PCR was carried out with the SYBR Green PCR kit (Takara Biotechnology, Japan) and sequence detection was performed using an ABI 7500 Sequencing Detection System (Applied Biosystems, Foster City, CA)

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A small randomized, double-blind trial that compared a high-fat with a high-carbohydrate hypercaloric diet and an isocaloric control diet reported more adverse events during the 6-months high-fat hypercaloric diet, including weight loss (167)

On the other hand, exclusion criteria included (1) review articles with poor experimental data, (2) research studies without clear methodologies, (3) research articles unrelated to pharmaceutical prospect of antioxidants and (4) publications without scope of our research work, (5) non-peer-reviewed studies, (6) literature older than 25 years, as well as (6) unpublished work