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To activate Cre recombinase and induce Gclc deletion in tumour pieces from Gclc f/f Rosa26 creERT2 MMTV-PYMT mice, female mice were intraperitoneally injected with tamoxifen (Sigma-Aldrich, T5648) at 50160 mg kg 1 once daily for 5 consecutive days

Automated single-particle data acquisition was performed with the EPU (Thermo Fisher Scientific) software at a nominal magnification of 105,000 , yielding a final pixel size of 0.83

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