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10.3389/fphar.2021.751380 Summary Keywords trimethylamine oxide, heart failure, chronic kidney disease, cardiorenal syndrome, trimethylamine Citation Zhang J, Zhu P, Li S, Gao Y and Xing Y (2023) From heart failure and kidney dysfunction to cardiorenal syndrome: TMAO may be a bridge

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Reaction products were purified by flash column chromatography on silica gel 60 (230400 mesh, Macherey Nagel) or aluminium oxide (activated neutral, Sigma-Aldrich) using a CombiFlash NextGen System and a preparative HPLC Quaternary Gradient 2545 equipped with a photodiode array detector 2998 (Waters) fitted with a reverse-phase column (XBridge BEH C18 OBD prep column 5 m, 30 150 mm)