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The slides were washed in water and then blocked using 10% goat serum in PBS for 1 h at room temperature before adding primary antibodies diluted in the blocking buffer

Another explanation could be that these adducts may break into lactone and the substrates (for example, secondary amines) of former Micheal-type addition reaction, due to the reversibility of this type of reaction (Lawrence et al., 2001)

However, it does not show any signs of oxidation on the selenium atom of U46, unlike what has been reported recently for a U46-containing GPX4 protein produced via a different strategy 49