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Amplicons were gel-purified, and amplified by PCR with primers 2514A and 2514 BamHI D

It is presumed that more than 600 types of E3 ubiquitin ligases exist in the human genome and that each one of them recognizes specific substrates, suggesting that different types of ubiquitin-conjugation reactions play key roles in regulating a variety of cellular events, including signal transduction, gene transcription and the cell cycle 5

PGC-1 is also involved in glucose and lipid metabolism, and has become a new target for the treatment of diabetes, obesity, and other metabolic diseases 20

It is essential for nerve health, red blood cell formation, and DNA synthesis