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For the protein kinase deletion mutant screen, strains were grown in 1 mL liquid YPD medium using a 24-well plate and incubated for overnight at 30 C with shaking at 180 rpm

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For all experiments, Sham control mice were age-matched GD3s / mice that did not undergo any surgical procedure or antibody injection

The bidirectional communication between your gut microbiome and brain means that alcohol-induced gut damage can simultaneously impair both your mental health and your bodys detoxification processes