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The rabbits were sacrificed 4, 8, and 12 weeks after intra-articular MSC injection to examine the regeneration of cartilage defects

Immunofluorescence (IF) ESCs were fixed with 4% paraformaldehyde at 4 C for 10 min and then permeabilized with 5% Triton-100 at room temperature for 30 min

In cases of benign nodules, a fine needle aspiration (FNA) is usually conducted to assess the nature of the nodule, while RFA or ethanol injections are performed to shrink benign nodules or for symptomatic improvement