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While there is nothing wrong with including covariates in studys design, many researchers will include one only after their initial analysis (without the covariate) was found to be non-significant

At 450 nm, the optimum concentration is when the difference between positive and negative absorbance ratio (P/N) is the largest, and the positive value is close to 1 at the same time

One end was connected to the needle of a syringe full of air, and the acceptor phase containing sulfadiazine was then flushed out from the fiber lumen into a clean glass liner tube (250 L, Alltech, Deerfield, IL, USA)