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4T1 cells were stained with 2,7-dichlorofluorescein diacetate (DCF-DA), a typical probe of ROS 40 , under different treatment conditions

FIGURE 1 Mechanisms of innate immunity in regulating bone metabolism: shared pathways Across the aforementioned innate immune components (immune cells, complement system), several core pathways are repeatedly involved in bone metabolism regulation, forming a shared regulatory network: 1) NF-B signaling pathway: activated by pro-inflammatory cytokines (TNF-, IL-6) or complement products (C5a), it promotes osteoclast differentiation via upregulating NFATc1