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We have quite a few additional articles discussing glutathione agarose beads and GST affinity purification, and you can find links to those at the end of the article

Additionally, non-pharmacological interventions such as cognitive training, cognitive behavioural therapy, and art or light therapy, previously deemed exotic, are now beginning to be integrated into therapeutic guidelines

Other pharmacological options include catechol-O-methyltransferase (COMT) inhibitors like entacapone and opicapone, which extend the half-life of levodopa, do not cross the BBB at all and primarily affect peripheral metabolism, providing no direct action in the brain 59

Plasmids and lentivirus production Plasmids The all-in-one CRISPRi plasmid encoding dCas9-KRAB and sgRNA were obtained from Addgene (catalogue no