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J Clin Endocrinol Metab 88 : 34503453 Date Y et al

Store the mixed vial in the refrigerator and draw doses with an insulin syringe

HEK293 cells transfected with SNAP-ghrelin receptor (WT or mutants) were seeded at a density of 1 10 6 cells into 3 cm dish and incubated for 24 h at 37 C in 5% CO 2