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To construct the pPapi-SLC25 library, SpCas9 and SaCas9 guides were designed to target 53 SLC25 carriers, seven additional SLC proteins present in MitoCarta 2.0 16 , BCL2L1, MCL1 (the two paralogous anti-apoptotic proteins) and EEF2 (elongation factor 2, essential for protein translation) as positive controls, 8 non-cutting controls and 19 cutting controls that target olfactory receptors (ORs) as negative controls

Complementary evidence comes from Wang et al., who used cadmium-induced testicular toxicity model in male rats and showed that quercetin mitigated oxidative damage in the testes by evaluating SOD, GPx, CAT and GSH, lowering MDA levels, and reducing cadmium accumulation