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The synthesis of isotope containing cleavable biotin tags [L-Valine-N-Fmoc ( 13 C 5 , 15 N 1 )] is usually expensive and low yield, but recently, a more cost-effective version of light and heavy isotopes of IA-alkyne tags (Figure 5C) has been developed ( Quantitative Thiol Reactivity Profiling (QTRP) In a strategy similar to isoTOP-ABPP, a quantitative thiol reactivity profiling (QTRP) approach was introduced for Oxi-proteomics analysis in which IAM-alkyne probes are used together with click chemistry for light and heavy isotope labeling ( N -(prop-2-yn-1-yl)acetamide, IPM, Figure 5C) coupled with isotopically light ( 12 C) and heavy ( 13 C) UV-cleavable azido-biotin tags via a click chemistry reaction (CuAAC) (Figure 8)

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investigated serum GABA, glutamate (Glu), and the GABA/Glu ratio in children with ASD and found that plasma GABA, Glu, and the GABA/Glu ratio were significantly elevated in children with ASD (120)