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Growth rates were predicted by setting the biomass reaction in the iCHO2291 model as the objective function and applying enzymatic capacity constraint flux balance analysis (ecFBA), which, unlike conventional FBA (e.g., FBA, FVA, pFBA), integrates enzyme kinetic constraints like turnover numbers ( k cat ) alongside standard FBA constraints, enhancing the accuracy of intracellular flux distributions by accounting for enzyme efficiency and enabling exploration of metabolic resource allocation 33

During the early stages of AS, moderate macrophage death reduces inflammation and metalloproteinase secretion

doi: 10.1136/bmj.n71 118 PaivaA

NL and BC purified proteins and BC assisted with DsbA in vitro assays