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Membranes were blocked with 5% skim milk in TBST (0.1% Tween-20) prior to overnight incubation (4 C) with specific primary antibodies: CCR1 (1:1000, Origene, TA374392, MD, USA), TNF- (1:500, Bioworld, BS1857, Nanjing, China), IL-1 (1:1000, Abcam, ab283818, Cambridge, UK), ZO-1 (1:1000, Invitrogen, 33-9100, CA, USA), Occludin (1:1000, Invitrogen, 33-1500, CA, USA), UPK3 (1:1000, PROGEN, 690108S, Heidelberg, Germany), FOXO1 (1:1000, Proteintech, 18592-1-AP, Wuhan, China), p-FOXO1 (1:1000, Affinity, AF3416, Changzhou, China), PPAR (1:1000, Proteintech, 66826-1-Ig, Wuhan, China), PPAR (1:1000, Proteintech, 81490-5-RR, Wuhan, China), PPAR (1:1000, Proteintech, 60193-1-Ig, Wuhan, China), GAPDH (1:1000, CST, 2118S, MA, USA)

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Autoimmunity Autoimmune diseases occur when the immune system mistakenly attacks healthy cells, leading to chronic inflammation, tissue damage, and immune dysfunction

BPC157 was added at concentrations of 5, 10, 20, and 40 g ml-1 for the indicated periods