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These approaches ensure a 1 nM effective concentration of the fluorescently labeled nanobody (NbLumSyt1-pH/Nb-At647N or NbLumSyt1-HALO/JF549) on the imaging plate, allowing partial labeling of the Syt1 nanobodies without over-saturating the fluorescent signal enabling single molecule detection

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B12 Injections: Are They Safe and What Should You Expect