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A placebo-controlled, cross-over study of vitamin and mineral supplementation, for example, suggested some improvement in the treatment group, but statistical analysis indicated that the effect of treatment was not significant.[31] A study of intramuscular bovine liver extract containing folic acid and cyanocobalamin showed no improvement compared with placebo.[32] A number of dietary theories have been put forward over the years, often based on anecdotal evidence, but a review of some of these asserted that there was little evidence to support them, for example the use of yeast-avoidance and sugar-free diets, to combat Candida albicans infection

Enzyme-linked immunosorbent assay (ELISA) For the ligandreceptor affinity ELISA, the human FBXO22 protein served as an antigen to coat the 96-well plate at 4 C for 24 h

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