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After intensive washing with PBS, endogenous peroxidase activity was blocked by incubation of the slides in a 3% hydrogen peroxide solution

If youve read our other articles on affinity tags or affinity purification of antibodies, then you already know that affinity purification involves three key steps: 1) binding the target protein to the beads, 2) washing away contaminating proteins, and 3) eluting your protein of interest (Figure 3)

These responses include activation of mitochondrial biogenesis, fiber type transformation and angiogenesis

10.1016/j.phrs.2015.06.006 102 LuoX.BaoX.WengX.BaiX.FengY.HuangJ.et al (2022)