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Cells were fixed with 4% paraformaldehyde for 20 min at room temperature, and then permeabilized with 0.3% Triton X-100 for 10 min at room temperature

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Nevertheless, a significant number of AAS products are expected to be analyzed in 2024 because illicit sites that distribute huge quantities of anabolic agents (located in Poland) have recently been tracked down by the police ( Conversely, the number of other PED samples increased (mean 21.1%) from 7.5% in 2020 to 32.9% in 2023