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Castellini C, Mattioli S, Dal Bosco A, Collodel G, Pistilli A, Stabile AM, et al

For the estradiol treatment, 0.3 cm silastic estradiol capsules (Baxter HealthCare, Mundeleine, IL, USA) were implanted subcutaneously in the mice

I notice balls get bigger and load increases a lot

For SidC-mediated ubiquitination reaction 49 , 0.3 g GST-E1, 1 g His 6 -UbcH7, 3 g GST-SidC 1-542 and 4 g Ub were incubated in a 20 L reaction system containing 50 mM Tris-HCl (pH 7.5), 5 mM MgCl 2 , 1 mM DTT and 2 mM ATP for 1 h at 37 C