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When IB formation is a problem, recombinant protein synthesis should be carried out in the range 1525C, though one report described successful expression at 4C for 72 h (San-Miguel et al., 2013)

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Other researchers have directly loaded therapeutic molecules into exosomes using drugs or gene tools, creating customized exosomes with enhanced neuroprotective, anti-inflammatory, or angiogenic properties, including GPX4 activators, clustered regularly interspaced short palindromic repeats (CRISPR)/Caspase-9, and specific miRNA overexpression methods (53, 55, 57, 58)

As the clinical evidence section highlighted, there are now successful examples (e.g., the QLQX heart failure trial, and growing data on berberine)