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Placental growth factor (PlGF), another member of the VEGF family, is an important regulator of the pro-angiogenic phenotype within the TME

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Human NSCLC cell lines, A549, Lu99 and EBC-1, (Riken BioResearch Center, Tsukuba, Japan) were cultured in 100-mm dishes (Becton Dickinson Labware) in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) (Moregate Biotech), penicillin (5 g/ml), streptomycin (5 g/ml) and neomycin (10 g/ml)