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Hidayat, S

polynucleotide shuffling & interrupted synthesis ' ) and non-stochastic polynucleotide reassemblv methods of the invention provide means for optimizing DNA sequences and the three-dimensional structure of the plasmids for ability to confer upon a vector the ability to enter a cell even in the absence of detailed information as to the mechanism by which this effect is achieved The methods involve reassembling (&/or subjecting to one or more directed evolution methods described herein) at least first and second forms of a nucleic acid that comprises a transfer sequence

E.GaoX.DixonH.McDonoughJ

Jansen KL, Faull RL, Dragunow M, Synek BL