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Researchers confirmed this through knockout studies in mice, where animals lacking beta-3 adrenergic receptors showed zero response to AOD 9604, proving the peptide works specifically through this receptor pathway in adipose tissue

This information ensures consistent results and safe handling in research settings

For SH-SY5Y/MYCN, the culture media was supplemented with 7.5 g/mL Blasticidin (Bio-Sciences) and 200 g/mL Geneticin (G418) (Thermo Scientific) for selection of the plasmid