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Front Endocrinol (Lausanne) 12:718235 Stancill JS, Broniowska KA, Oleson BJ, Naatz A, Corbett JA (2019) Pancreatic -cells detoxify H(2)O(2) through the peroxiredoxin/thioredoxin antioxidant system

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After qPCR quantification using the KAPA library quantification kit (Roche), Sequencing was carried out on the NovaSeq 6000 instrument from Illumina based on a 2 100 cycle mode (paired-end reads, 100 bases) to obtain around 100 million clusters (200 million raw paired-end reads) per sample

Notably, we previously reported that HrcU C interacts in vitro with the early T3S substrate HrpB2 but not with the putative translocon protein XopA or the effector protein XopC, suggesting that HrcU C is not a general T3S substrate docking site (Lorenz and Bttner, 2011)