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Proteomics analysis Label-free LC-MS/MS was used for proteomics analysis of the blank group and D-CuP group (n = 3 in each group)

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Just like Ator1, AtorC makes several interactions with the S4S5 linker and the S4 and S5 helices, but because these helices have different relative locations in the open and closed states, AtorC is tilted relative to Ator1 to avoid clashes with the S4S5 linker and S4 helix in the closed state (Fig