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J Clin Endocrinol Metab 1992

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For example, pregnant women might need more folate, while older adults might need more calcium and vitamin D

After the addition of three sample volumes of ice-cold methanol, mixing and centrifugation (20,000 g , 10 min, 4 C), the supernatant was removed and the pellet washed with three sample volumes of ice-cold methanol, mixed and centrifuged (20,000 g , 10 min, 4 C), followed by air-drying for around 10 min