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Research is investigating biomarkers that could predict responsiveness to NAD+ enhancement, optimal dosing strategies for different applications, and combination approaches that pair NAD+ precursors with other interventions for synergistic effects

Collected villi were counted, and 100 villi were suspended in 20 l of either collagen or Matrigel matrix and plated into 24-well plates

Washed membranes were incubated with a secondary antibody conjugated with horse radish peroxidase, anti-mouse (1:1000, Abcam, ab7064), or anti-rabbit (1:1000, Abcam, ab72465) in TBST for an hour at room temperature

Further details can be found in our vitamin B12 deficiency charts