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In secondary hypothyroidism the TSH is unreliable, and doses should be titrated to the T4 level Advise patients: Treatment is usually lifelong There is an association with other autoimmune diseases, particularly: Pernicious anaemia Addisons disease About the risk of side effects Typically few, and usually dose related due to too much thyroxine causing signs of hyperthyroidism: Palpitations and atrial fibrillation Diarrhoea Increase appetite Anxiety Insomnia Tremors Subclinical hypothyroidism Historically, a contentious issue Defined as elevated TSH, with normal T4

10.1172/JCI24586

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FIGURE 4 WGS metagenomics and traditional metagenomics (e.g., amplicon sequencing of marker genes like 16S, 18S, or ITS) both aim to profile microbial communities, but they differ fundamentally in scope and resolution