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For mouse brains injected with AAV vectors, brain tissue was isolated and homogenised in a buffer composed of 20 mM HEPES-NaOH (pH 7.4), 150 mM NaCl, 0.5% Triton X-100, and complete protease inhibitor cocktail (EDTA-free) and sheared with a 23-G and then by a 27-G needle syringe with agitation for 1 h at 4 C

In OS conditions, the capacity of RBCs to reduce GSSG to GSH decreases, leading to GSSG accumulation and, consequently, to GSH depletion [14]