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Description

ICH, a severe subtype of stroke, is characterized by a complex pathological process, with no specific treatments currently available

Site-directed mutagenesis was performed using a QuikChange kit (Agilent) and a primer pair (Supplementary Table 3) to incorporate an upstream ribosomal binding site

Reducing agent incompatibility: Any buffer or medium containing DTT, TCEP, or beta-mercaptoethanol will reduce the Cys2-Cys7 disulfide bridge, inactivating the peptide