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Histologic lesions of the lower GI tract consisted of multifocal areas of mucosal erosion denuded of epithelial cells, reduction in goblet cells, dilated crypts, crypt collapse, submucosal edema, and transmural distribution of mixed inflammatory infiltrates

GST-OLFM4 protein was expressed in BL21 (DE3) by 0.5 M IPTG inducing overnight, then harvest cells and resuspend in phosphate-buffered saline (PBS) (0.1% Triton100, Protease Inhibitor Cocktail, 1 mM PMSF), after lyzed by sonication, proteins were purified by glutathione Sepharose 4B (GE healthcare)