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(B) pcDNA3.1-V5_GSTP1-WT, -Cys15Ser, -Cys48Ser, -Cys102Ser or-Cys170Ser were transiently transfected in HEK293T cells, metabolically labelled with -alkynyl-palmitate (+) or treated with DMSO (-), immunoprecipiated with mouse anti-V5 antibody (V5-10) conjugated to agarose beads and subjected to click chemistry

Increased Expression Levels The GST tag also facilitates higher levels of protein expression

This lack of sensitivity can lead to the conclusion that the prolonged symptoms reported in Lyme disease are no different than the background noise of symptoms in the general population