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Analysis using electron microscopy have shown that 12 h after exposure to HgCl 2 , the cells of the renal tubules had ruptured plasma membranes, loss of microvilli, decreased contact with the basement membrane and destruction of the cell morphology [34]

Equal amounts of protein were subjected to SDS-PAGE and transferred onto polyvinylidene fluoride membranes

Statistical analysis Data were presented as means standard deviations

In addition, despite great progress regarding the role of nonsense-mediated decay of SCN1A in disease modification [170, 171], there is still no approach to modify epilepsy in clinical practice