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10.3389/fmed.2023.1157773 319

BBR demonstrated bacteriostatic effects on both S

Materials : water (50 mL), ethanol (50 mL), peristaltic pump, water bath, Falcon tube holders (9-tube and 16-tube), Vacutainer Safety-Lok Blood Collection Set, syringes (1 mL), 27 G needles, paper roll, tray, tape, and various reagents including calcium and magnesium-free EBSS (Gibco, 12539069), EGTA, HEPES, Williams E medium (Gibco, 11570606), L-glutamine, penicillin/streptomycin, fetal bovine serum (FBS), collagenase type IV (Thermo, 17104019), trypsin inhibitor (Merck, T6522), heparin, ketamine, and xylazine

Indeed, glutathione production studies focused of genetic engineering reported about 10300 mg/L of glutathione production [32] and the reason for these low yields may be the not optimized culture conditions