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This is usually due to a lack of bioavailability

Four different approaches can be used for priming cDNA reactions in two-step assays: oligo(dT) primers, random primers, or sequence specific primers ( Figure 2 and Table 2 )

Guo C, Lu H, Gao W, Wang L, Lu K, Wu S, Pataer A, Huang M, El-Zein R, Lin T, Roth JA, Mehran R, Hofstetter W, Swisher SG , Wu X, Fang B

Perhaps, one of the most relevant characteristics of cachexia is fatigue, which reflects the extensive muscle wasting associated with syndrome [5, 7, 8]