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However, this marker is not completely clear because HMNT is only responsible for about 70% of histamine degradation the rest is done by the enzyme DAO

Production of serotonin Figure 4 , is critically dependent upon the action of the enzyme Tryptophan-5-Hydroxylase (TPH), followed by the subsequent reaction of the PLP dependent enzyme, Hydroxy-Tryptophan Decarboxylase (HTD)

Each method has its own benefits and is suited for different needs