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Updated 10/18/2020

4.1.2 Bile acid metabolism Bile acids (BAs) serve as pivotal mediators in microbiota-host crosstalk, with their biotransformation from primary to secondary forms critically dependent on gut microbiota

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To address this, we isolated primary mouse hepatocytes and infected them with adenoviruses expressing Chac1 or GFP, followed by treatment with BSA or BSA-conjugated fatty acids to mimic chow and HFD conditions, respectively