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therefore, we mainly focused on loops 3 and 5 to investigate if their sequence variations are linked to their distinctive substrate differences

HL, LC, and BR: drafting the work

Research has demonstrated that excessive ROS can ultimately result in both single-stranded and double-stranded DNA damage, impairing cell proliferation and intercellular adhesion, which may contribute to genomic instability and cell death (Sehitogullari et al., 2014)