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Hysterectomy is performed under general anesthesia (isoflurane 2%) at the end of the treatment and the two uterine horns are placed in physiological Krebs' solution until isometric tension measurements within no more than 1 h

In astrocytes, Cu toxicity is associated with oxidative stress, as treatment with antioxidants mitigates Cu-induced cell death, demonstrating that oxidative stress mediates Cu-induced astrocyte cytotoxicity (Gale et al., 2023)