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doi: 10.1007/BF00507974 49 KohnABAndersonPAVRoberts-MisterlyJMGreenbergRM

Peripheral blood mononuclear cells were isolated by Ficoll density gradient centrifugation and monocytes were isolated by plastic adherence by culturing 1 10 6 cells/mL for 2 h in serum-free media at 37 C [20]

Acetylation spatial regulation An alternative strategy for controlling enzyme selectivity and activity is through spatial confinement, which enables acetylation to occur preferentially on proteins within specific compartments such as the nucleus, cytoplasm, mitochondria, and endoplasmic reticulum