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The quality of bowel preparation was evaluated in the two patient groups

The pellet was resuspended in 150 L of 50 mM Tris-HCl buffer (pH 7.5), 100 mM NaCl, 0.1% NP-40 and the resulting PEDF-R-containing preparation (50 L) was incubated with 5 M PEDF and L--phosphatidylcholine (# P5638, Sigma-Aldrich, USA) in 50 mM Tris-HCl (pH 7.5), 3 mM deoxycholate with or without 50 M ZnCl 2 in a final volume of 0.5 mL at 37 C for 20 min

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