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This can be attributed to the increase in the net negative and positive charges as the pH deviates from the isoelectric point, which promotes intermolecular repulsion, enhances the dispersive ability, and ultimately increases protein solubility (32)

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Chemicals for HPLCMS/MS 5,5-Dithiobis-2-nitrobenzoic acid (DTNB), methanol, acetonitrile, 1 mM ammonium acetate, 0.1 % TFA, and formic and acetic acid were purchased from Sigma-Aldrich

465,466,467,468 In RA, mitochondrial dysfunction drives chronic synovial inflammation despite preserved mitochondrial mass