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Transmission electron microscopy (TEM) revealed an increase in autophagosomes, while immunofluorescence analysis showed enhanced colocalization of LC3B with MitoTracker-labeled mitochondria (marking mitophagosomes) in M9a+sh EPAS1 cells, further indicating enhanced mitophagy (Fig

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However, several conditions can increase the susceptibility to acetaminophen hepatotoxicity, such as malnutrition, chronic illness, and chronic ethanol ingestion, and the toxic dose of acetaminophen may be lower in these patients