glutathione and sulforaphane Metabolism of 4-methylsulphinylbutyl glucosinolate Frontiers | Glutathione: A Samsonian

Nature 458 , 719724 (2009)

C57BL6/J WT (Gstp1/2 +/+ ) mice and Gstp1/2 knockout (Gstp1/2 / ) mice in C57BL6/J background were provided by Professor Roland Wolf and Cancer Research UK

3 Results and Discussion 3.1 Fragment Screening: 12 Hits Identified in 5 Sites Crystallographic fragment screening performed at the XChem facility allowed us to test 352 small compounds from the DSiP library, a collection specifically designed to ensure rapid and cheap follow-up synthesis ( Data analysis, performed by the PanDDA algorithm (Figure 1), described later in detail

Afterwards, fresh media containing MSCm, NanoSU6668, MNS and T-MNS (25 M) was added to the plates, and cells were incubated continuously for 12 h